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C.86-Breast Cancer And Virus: Molecular Homology Between Human Mammary Tumor Virus 3’Orf And Scorpio PDF print email
Written by TRAN Guy Mong Ky   
Monday, 09 December 2013 12:27
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C.86-Breast Cancer And Virus: Molecular Homology Between Human Mammary Tumor Virus 3’Orf And Scorpion Toxin, A Ligand Of Voltage-Gated Sodium Na+ Channel. Omega 3, A Na+ Channel Modifier, Reduces The Risk Of Metastatic Breast Cancer.

Présentation Poster au 5° congress européen de virology (Lyon Septembre 2013) REF 017 Category: 03.Innate immunity against viruses

Breast cancer has multiple etiologies: Many virus were implicated [Epstein-Barr virus, Human oncogenic Papillomavirus, Adenovirus, Simian Virus 40 (SV40)]. Some discrepancies (Park DJ, 2011) can be relevant to this multiplicity of viruses. Other factors can be unrecognized: Genetics, radio-activity, aluminium, genetically modified organisms.


The human homologue of Mouse Mammary Tumor Virus (MMTV) (Bittner JJ, 1936), re-called

Human Mammary Tumor Virus (HMTV), was found in human Breast Cancer (BC), in variable % of cases:  74% in Tunisia, 42% in Australia, 38% in Italy, 36% in United States, 31% in Argentina, 0.8% in Vietnam (Levine PH, 2004). This geographical disparity may depend on various factors, such as the degree of mouse infection by MMTV (Stewart TH, 2000; Szabo S, 2005). The host susceptibility or resistance to MMTV is depending on the species genetic background, particularly the T Cell Receptor (TCR) Vbeta.

The MCF-7 and MDA-MB-231 breast cancer cell lines were demonstrated to be HTMV-positive (Wang Y, 1995); when injected in nude mice, they induce metastatic breast cancer (Shafie SM, 1980; Price JE, 1990). In 123 treated breast cancers, Bougnoux P (1995) found after 48 months 70% metastasis if the breast fat alpha-linolenic acid (omega-3 precursor) level was low (9% if it was high): Thus, omega-3 (18:3n-3) protects against metastasis.



As omega-3 modifies the voltage-gated Na+ channel (NaCh) (Xiao YF, 2001; Banu I, 2006), we looked for a NaCh ligand (scorpion toxin) in HMTV.

METHODS: Amino Acid (AA) sequence comparison.

RESULTS: we found a molecular homology between HMTV 3’orf and scorpion toxin chimera (Possani LD, 2000)/Euscorpius Flavicaudus (AAT76439) (58-71) implicated in development:



We demonstrated the HMTV oncogenicity by the discovery of 2 major oncogenes in the 3'ORF:

Mdm2, the p53 ligand (Tran GMK, 2004) and Notch-1 (Tran GMK, 1998). Furthermore,  MMTV is a hormonal virus, because it integrated upstream of and activated Aromatase (Int-5), the estrogen synthetase (Tekmal RR ,1997).





HTMV has 2 major oncogenes (mdm2, the p53 ligand, and Notch-1) and is a hormonal virus integrating upstream of and activating aromatase, the estrogen synthetase. HTMV 3’orf contains a scorpion toxin, explaining the metastasis protection conferred by omega-3, a NaCh blocker.The omega-3-rich soya diet in Asia, added to the low HTMV frequency in Asia (Japan, China, Vietnam), may explain the low asian breast cancer prevalence. We suggest to use omega-3 in association with  chemotherapy in metastatic breast cancer; and study other second generation NaCh ligands (Tran GMK, Allostery EMBO Conf 2013; Djamgoz MBA, 2006).

Green tea (Yang CS, 2010) associated with mushrooms were also very interesting alicaments (breast cancer risk reduction of 89%, with an odds ratio = 0,11 (Zhang M, 2009). Corossol (graviola) was re-assessed: It down-regulates EGFR expression (Dai Y, 2011).


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